HiPure Circulating DNA Mini Kit, designed to efficiently purify short, cell-free DNA (cfdna) molecules from human biological fluids:

• Target:
  • CFDNA-Fragmente und mRNAs im Blut von Tumors abgeleitet
  • Fötus -Nukleinsäuren im mütterlichen Blut
• Sample Compatibility:
  • Menschliches Plasma
  • Menschliches Serum
  • Menschlicher Urin
  • Samples can be fresh or frozen (once only)
• Konzentrieren Sie sich auf kurze DNA: The kit isolates short nucleic acids (<1000bp) häufig in Biofluiden gefunden.
• Effiziente Reinigung: The protocol allows for effective recovery of cfDNA from various biofluids.
• Ready for Downstream Use: Purified cfDNA is suitable for diverse applications, einschließlich:
  • PCR and other amplification reactions
  • Sequenzierung der nächsten Generation (NGS) analyses

Vorteile:

• Optimiert für kurze cfDNA: Specifically designed to capture short DNA fragments relevant for biomarker discovery and liquid biopsies.
• Breite Probenkompatibilität: Works effectively with various human biofluids, Flexibilität bei der Probenauswahl bieten.
• Streamlined Workflow: The miniaturized kit format allows for faster processing compared to larger kits.

This kit empowers researchers to study circulating DNA in various fields, including oncology, Nicht-invasive pränatale Tests (Schluck), and liquid biopsy applications.

Spezifikationen

Prinzip

This product utilizes silica gel columns for a rapid and reliable DNA-Reinigung process.

1. Lysis and Digestion: The sample undergoes treatment with a lysate solution and protease. This breaks open cells and degrades proteins, releasing DNA into the solution.

2. Binding to Silica Membrane: The lysate containing the DNA is transferred to an adsorption plate or filtration column. The silica membrane in these tools selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.

3. Waschen: Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.

4. Elution: A low-salt buffer (Typischerweise 10 mM Tris, pH 8.0) is used to elute the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.

Diese Methode bietet ein wertvolles Instrument für Forscher, die in verschiedenen Bereichen arbeiten, für die eine gereinigte DNA für die Analyse erfordern, such as PCR, Southern Blot, and next-generation sequencing.

Vorteile

• Hohe Ausbeute – optimalster Prozess, freie DNA (>50bp) kann maximal gewährleistet werden
• Hohe Konzentration – Niedriges Elutionsvolumen, Gewährleistung einer hohen Nukleinsäurekonzentration
• Hohe Reinheit – Niedrige Alkoholbindungsmethode, vollständig entfernen Inhibitor und Proteinverschmutzung
• Hohe Genesung – DNA kann auf der PG -Ebene durch Kieselgeläuschenreinigung gewonnen werden

Inhalt des Kits

Lagerung und Stabilität

Here’s a quick guide on storing your DNA extraction kit to ensure it functions at its best:

Ideal Storage:

• Store Proteinase K and Carrier RNA in the refrigerator (2-8°C) upon arrival for maximum effectiveness.

Acceptable Short-Term Storage:

• Proteinase K and Carrier RNA can be kept at room temperature (15-25°C) for up to 12 weeks without impacting their performance.

Storing Remaining Components:

• All other kit components should be stored dry at room temperature (15-25°C).
• They will remain stable for at least 18 Monate unter diesen Bedingungen.

Important Notes:

• If storing the entire kit at 2-8°C, remember to redissolve buffers before use.
• Always ensure all buffers reach room temperature before using them for optimal results.

By following these storage recommendations, you can guarantee your DNA extraction kit is always ready for use and delivers the best possible results.