HiPure Mikro-Kit für zirkulierende Nukleinsäuren

HiPure Mikro-Kit für zirkulierende Nukleinsäuren, is designed to efficiently purify short nucleic acids (<1000bp) from human biological fluids.

• Target Molecules:
  • Tumor-derived cell-free DNA fragments (cfdna) and mRNA in blood
  • Fötus -Nukleinsäuren im mütterlichen Blut
  • Free-circulating cell-free RNA (cfRNA)
  • Viral nucleic acids
• Sample Compatibility:
  • Menschliches Plasma
  • Menschliches Serum
  • Menschlicher Urin
  • Samples can be fresh or frozen (once only)
• Effiziente Reinigung: The kit isolates short nucleic acids present in biofluids.
• Hohe Reinheit: Purified nucleic acids are free of proteins, nucleases, and other contaminants.
• Downstream-Anwendungen: The eluted nucleic acids are ready for use in various downstream analyses, einschließlich:
  • Amplification reactions (z.B., PCR)
  • Long-term storage (-30°C to -15°C)

Vorteile:

• Suitable for Short Nucleic Acids: Specifically designed to capture short fragments often found in biofluids.
• Breite Probenkompatibilität: Works effectively with various human biofluids.
• High-Quality Results: Delivers purified nucleic acids free of impurities, ensuring reliable downstream applications.
• Convenient Storage: Purified nucleic acids can be stored for long-term use.

This kit empowers researchers studying circulating nucleic acids in various fields, including oncology, Nicht-invasive pränatale Tests (Schluck), and viral diagnostics.

Spezifikationen

Prinzip

This product offers a fast and efficient method for purifying DNA using silica column chromatography. Here’s a breakdown of the process:

1. Lysis and Digestion:

• The sample is treated with a lysate solution and protease to break open cells and degrade proteins. Dies setzt DNA in die Lösung frei.

2. Binding to Silica Membrane:

• The lysate containing the DNA is transferred to an adsorption plate or filtration column. The silica membrane in these tools selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.

3. Waschen:

• Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.

4. Elution:

• A low-salt buffer is used to elute the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing collection of purified DNA in a separate tube.

Diese Methode bietet ein wertvolles Instrument für Forscher, die in verschiedenen Bereichen arbeiten, für die eine gereinigte DNA für die Analyse erfordern, such as PCR, Southern Blot, and next-generation sequencing.

Vorteile

• Effiziente Reinigung: Silica column technology offers a streamlined process for isolating DNA from complex biological samples.
• Hochwertige DNA: The selective binding properties of the silica membrane ensure the purification of high-purity DNA, suitable for various downstream applications.
• Vereinfachter Arbeitsablauf: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.

Inhalt des Kits

Lagerung und Stabilität

Optimal Performance:

• Store Proteinase K and Carrier RNA at 2-8°C (Kühlschrank) upon arrival for best results.

Acceptable Short-Term Storage:

• Proteinase K and Carrier RNA can be stored for up to 12 Wochen bei Raumtemperatur (15-25°C) ohne ihre Leistung zu beeinflussen.

Verbleibende Kit -Komponenten:

• Store all other components of the kit dry at room temperature (15-25°C).
• Sie sind zumindest stabil 18 Monate unter diesen Bedingungen.

Important Notes:

• If storing the entire kit at 2-8°C, Stellen Sie sicher, dass Puffer vor dem Gebrauch wieder aufgelöst werden.
• Always warm all buffers to room temperature before using them for optimal performance.

By following these storage guidelines, you can ensure the longevity and effectiveness of your DNA extraction kit.